Installing VAT

Q:
Thank you for offering VAT free to scientific community. A few users of our cluster requested this software to be installed on our 6000 node cluster.
But I ran into problem while trying to compile it. Hopefully you can provide some help.Thank you in advance.

I have followed your instruction on
http://vat.gersteinlab.org/documentation.php
But when I try to make vat, I got following errors. I have attached the
config.log file.:

/data/rl/vat-2.0.0> env | eg FLAGS

LDFLAGS=-L/usr/local/gsl-1.14/lib -L/usr/local/gd-2.0.35/lib
-L/usr/local/libs3-2.0/lib -L/usr/local/bios/lib
CPPFLAGS=-I/usr/local/gsl-1.14/include -I/usr/local/gd-2.0.35/include
-I/usr/local/libs3-2.0/include -I/usr/local/bios/include

/data/rl/vat-2.0.0> make
make all-recursive
make[1]: Entering directory `/gs1/users/rl/vat-2.0.0′
Making all in lib
make[2]: Entering directory `/gs1/users/rl/vat-2.0.0/lib’
GEN alloca.h
GEN configmake.h
GEN langinfo.h
GEN stdlib.h
GEN unistd.h
GEN wchar.h
GEN wctype.h
make all-recursive
make[3]: Entering directory `/gs1/users/rl/vat-2.0.0/lib’
make[4]: Entering directory `/gs1/users/rl/vat-2.0.0/lib’
CC localcharset.o
AR libgnu.a
make[4]: Leaving directory `/gs1/users/rl/vat-2.0.0/lib’
make[3]: Leaving directory `/gs1/users/rl/vat-2.0.0/lib’
make[2]: Leaving directory `/gs1/users/rl/vat-2.0.0/lib’
Making all in src
make[2]: Entering directory `/gs1/users/rl/vat-2.0.0/src’
CC vcf.o
CC util.o
CC shutil.o
CC cfio.o
CC md5.o
CC growbuffer.o
CC s3.o
AR libvat.a
CC gencode2interval.o
CCLD gencode2interval
CC interval2sequences.o
CCLD interval2sequences
CC snpMapper.o
CCLD snpMapper
CC indelMapper.o
CCLD indelMapper
CC svMapper.o
CCLD svMapper
CC genericMapper.o
CCLD genericMapper
CC vcf2images.o
CCLD vcf2images
vcf2images.o: In function `generateLegend’:
/data/rl/vat-2.0.0/src/vcf2images.c:516: undefined reference to `gdImagePng’
vcf2images.o: In function `main’:
/data/rl/vat-2.0.0/src/vcf2images.c:564: undefined reference to `gdImagePng’
collect2: ld returned 1 exit status
make[2]: *** [vcf2images] Error 1
make[2]: Leaving directory `/gs1/users/rl/vat-2.0.0/src’
make[1]: *** [all-recursive] Error 1
make[1]: Leaving directory `/gs1/users/rl/vat-2.0.0′
make: *** [all] Error 2
/data/rl/vat-2.0.0> echo $?

A:
Thank you for your interest in VAT. It appears that the compiler is unable to find the reference for gdImagePng, which is part of the GD library.
It is important to install and configure the GD library before installing VAT.

problems in compilation of libproteingeometry-2-3-1

Q.
I am experiencing problems in trying to compile your package (libproteingeometry-2-3-1). I tried to compile it under Ubuntu-11.10 on a 32-bit VAIO centrino-based PC (gcc vers 4.6.1) and I got the following error message:

utypes.h:16:15: error: conflicting types for ‘float_t’
/usr/include/i386-linux-gnu/bits/mathdef.h:36:21: note: previous declaration of
‘float_t’ was here

Is it possible that this error is related CPPFLAGS settings?

I have also had difficulty compiling on an Intel i7 64-b machine under Ubuntu-11.10 with the same gcc version. This time the message was:

../src-lib/.libs/libproteingeometry: undefined reference to `sincos’ ../src-lib/.libs/libproteingeometry: undefined reference to `ceil’ ../src-lib/.libs/libproteingeometry: undefined reference to `atan2′ ../src-lib/.libs/libproteingeometry: undefined reference to `acos’ ../src-lib/.libs/libproteingeometry: undefined reference to `sin’ ../src-lib/.libs/libproteingeometry: undefined reference to `rint’ ../src-lib/.libs/libproteingeometry: undefined reference to `sqrtf’ ../src-lib/.libs/libproteingeometry: undefined reference to `pow’ ../src-lib/.libs/libproteingeometry: undefined reference to `sqrt’ ../src-lib/.libs/libproteingeometry: undefined reference to `floor’ collect2: ld returned 1 exit status

A.
This problem was resolved in a strange way. The package was compiled in the /usr/local dir of an Intel i5 machine, and all the compiled material was brought to the corresponding dir of an i7 computer. Almost the same had been done for a vaio 32-bit computer using a CD with the Ubuntu 9.10 version on another 32-bit system.

There may have been some collisions with environment flags set by the preceding compilation of other packages. In particular, Amber10 and Gromacs-4-5-5 had already been installed on the i7 and vaio.

Information in .root file

Q:
By using CNVnator, I managed to create the .root file but from there I can’t go any further because when I try to create the histograms, it seem to be working, but it never creates any files after it’s done.
A:
New information is added to the .root file you provided in the command line.
During next calculation step CNVnator will extract this information from the file.

To browse the content of the .root file you can start ROOT and open browser (type “new TBrowser”).

Please see http://root.cern.ch for details.

PGOHUM00000250821 probably not a pseudogene

Q:
This is supported as a protein coding gene based on transcript and genomic data in human, and homology data. The differences with the human reference assembly (insertions at nt 475-476 and nt 496-497 in the CDS) are supported by transcript data and alignment to the alternate (Celera) assembly. The mouse protein NP_758465.2 (Ppp1r9b, Entrez GeneID 217124) is the same length as the human protein (NP_115984.3) and 96% identical. The region where the mouse gene is located on chromosome 11 has the same genes in the same order as the location on human chromosome 17 where this gene is annotated.
A:
Thanks to Dr. Janet Weber from the Refseq project group for pointing this to us. PGOHUM00000250821 is most likely a protein-coding gene PPP1R9B. The erroneous annotations probably results due to either an error or difference in the canonical human reference genome. Please note that this locus is tagged for follow-up by the Genome Reference Consortium as a possible locus where the reference genome is incorrect (GRC Jira system as HG-191, http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human/issues/chr17/ ).

CNVnator license

Q:
Does your license allow to provide commercial services based on your program?

A:
Commercial services can use CNVnator for free provided that original software/developers/paper is credited/cited.

Alex Abyzov

***********************************************************
Department of Molecular Biophysics and Biochemistry,
Yale University, 260 Whitney ave., P.O. Box 208114,
New Haven, CT, 06520, USA
Phone: 1-(203)-432-5405
e-mail: abyzov@gersteinlab.org
URL: http://homes.gersteinlab.org/people/aabyzov
***********************************************************

Cow pseudogenes?

[tag sb]
Q:
We’re wondering if you happen to have a database for cow pseudogenes
A:
We haven’t done a Pesudopipe run on cow genome.
I see that the genome is available from Ensembl. You can download the code and run it. In theory,
Pseudopipe can be executed when the genome and the annotation files are a part of Ensembl. The code to run Pseudopipe can be downlaoded from
http://www.pseudogene.org/DOWNLOADS/pipeline_codes/