I am a research student working on protein structures using computational methods. I have used the tool StoneHinge to determine the hinge region residue and %protein rigidity for a protein. To confirm and report the significance of the putative hinge residue, I induced single residue mutations and noted the changes in the %packing rigidity. I was unable to find any significant changes. Therefore to confirm the importance of the putative hinge residue and effects of mutation on the hinge movement/rotation, is there any other tool?
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